Part:BBa_K3385057:Design
T1G3R_SglaA_glaA
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1858
Illegal EcoRI site found at 5753
Illegal EcoRI site found at 7181
Illegal XbaI site found at 1623
Illegal XbaI site found at 5784
Illegal SpeI site found at 1097
Illegal PstI site found at 893
Illegal PstI site found at 4015
Illegal PstI site found at 6591
Illegal PstI site found at 8325
Illegal PstI site found at 8926 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1858
Illegal EcoRI site found at 5753
Illegal EcoRI site found at 7181
Illegal NheI site found at 4892
Illegal SpeI site found at 1097
Illegal PstI site found at 893
Illegal PstI site found at 4015
Illegal PstI site found at 6591
Illegal PstI site found at 8325
Illegal PstI site found at 8926 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1858
Illegal EcoRI site found at 5753
Illegal EcoRI site found at 7181
Illegal BglII site found at 3870
Illegal BglII site found at 5392
Illegal BglII site found at 8423
Illegal BamHI site found at 1729
Illegal BamHI site found at 2157
Illegal BamHI site found at 4850
Illegal BamHI site found at 5652
Illegal BamHI site found at 5778
Illegal BamHI site found at 6329
Illegal XhoI site found at 548
Illegal XhoI site found at 735
Illegal XhoI site found at 963
Illegal XhoI site found at 6189 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1858
Illegal EcoRI site found at 5753
Illegal EcoRI site found at 7181
Illegal XbaI site found at 1623
Illegal XbaI site found at 5784
Illegal SpeI site found at 1097
Illegal PstI site found at 893
Illegal PstI site found at 4015
Illegal PstI site found at 6591
Illegal PstI site found at 8325
Illegal PstI site found at 8926 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1858
Illegal EcoRI site found at 5753
Illegal EcoRI site found at 7181
Illegal XbaI site found at 1623
Illegal XbaI site found at 5784
Illegal SpeI site found at 1097
Illegal PstI site found at 893
Illegal PstI site found at 4015
Illegal PstI site found at 6591
Illegal PstI site found at 8325
Illegal PstI site found at 8926
Illegal NgoMIV site found at 2566
Illegal NgoMIV site found at 5110
Illegal NgoMIV site found at 8313 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3398
Illegal BsaI site found at 3536
Illegal BsaI site found at 4225
Illegal BsaI.rc site found at 4718
Illegal SapI site found at 8814
Illegal SapI site found at 9123
Design Notes
Full construct made using non-overlapping primers, which can pose difficulties on PCR amplification and subsequently USER assembly.
Source
Sequences were obtained using aspgd.org[1] and SingalP-5.0[2].
References
[1] Cerqueira GC, Arnaud MB, Inglis DO, Skrzypek MS, Binkley G, Simison M, Miyasato SR, Binkley J, Orvis J, Shah P, Wymore F, Sherlock G, Wortman JR (2014). The Aspergillus Genome Database: multispecies curation and incorporation of RNA-Seq data to improve structural gene annotations. Nucleic Acids Res 42 (1); D705-10.
[2] SignalP 5.0 improves signal peptide predictions using deep neural networks. JosÊ Juan Almagro Armenteros, Konstantinos D. Tsirigos, Casper Kaae Sønderby, Thomas Nordahl Petersen, Ole Winther, Søren Brunak, Gunnar von Heijne and Henrik Nielsen. Nature Biotechnology, 37, 420-423, doi:10.1038/s41587-019-0036-z (2019)